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human dermal lymphatic endothelial cells (hlec) (hmvec-dlyad)  (Lonza)


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    Structured Review

    Lonza human dermal lymphatic endothelial cells (hlec) (hmvec-dlyad)
    SVEC (A, B) or <t>HLEC</t> (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.
    Human Dermal Lymphatic Endothelial Cells (Hlec) (Hmvec Dlyad), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hlec+(hmvec-dlyad)/human+microvascular+endothelial+cells/pmc05055472-45-0-12
    Average 90 stars, based on 1 article reviews
    human dermal lymphatic endothelial cells (hlec) (hmvec-dlyad) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Novel action and mechanism of auranofin in inhibition of vascular endothelial growth factor receptor-3-dependent lymphangiogenesis"

    Article Title: Novel action and mechanism of auranofin in inhibition of vascular endothelial growth factor receptor-3-dependent lymphangiogenesis

    Journal: Anti-cancer agents in medicinal chemistry

    doi:

    SVEC (A, B) or HLEC (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.
    Figure Legend Snippet: SVEC (A, B) or HLEC (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.

    Techniques Used: Western Blot, Control, Fluorescence

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    Article Title: Functional Analyses of the Bone Marrow Kinase in the X Chromosome in Vascular Endothelial Growth Factor–Induced Lymphangiogenesis
    Article Snippet: Commercially available HLEC (HMVEC-dLyAd) were purchased from Lonza.



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    Lonza human dermal lymphatic endothelial cells (hlec) (hmvec-dlyad)
    SVEC (A, B) or <t>HLEC</t> (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.
    Human Dermal Lymphatic Endothelial Cells (Hlec) (Hmvec Dlyad), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hlec+(hmvec-dlyad)/human+microvascular+endothelial+cells/pmc05055472-45-0-12
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    human dermal lymphatic endothelial cells (hlec) (hmvec-dlyad) - by Bioz Stars, 2026-09
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    Lonza human dermal lymphatic endothelial cells (hlec) hmvec-dlyad
    SVEC (A, B) or <t>HLEC</t> (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.
    Human Dermal Lymphatic Endothelial Cells (Hlec) Hmvec Dlyad, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Lonza hlec (hmvec-dlyad)
    SVEC (A, B) or <t>HLEC</t> (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.
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    Average 90 stars, based on 1 article reviews
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    Lonza hlec hmvec-dlyad
    SVEC (A, B) or <t>HLEC</t> (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.
    Hlec Hmvec Dlyad, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hlec+(hmvec-dlyad)/hlec++hmvec+dlyad+/10__1161_slash_atvbaha__110__214999-167-2-7
    Average 90 stars, based on 1 article reviews
    hlec hmvec-dlyad - by Bioz Stars, 2026-09
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    Image Search Results


    SVEC (A, B) or HLEC (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.

    Journal: Anti-cancer agents in medicinal chemistry

    Article Title: Novel action and mechanism of auranofin in inhibition of vascular endothelial growth factor receptor-3-dependent lymphangiogenesis

    doi:

    Figure Lengend Snippet: SVEC (A, B) or HLEC (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.

    Article Snippet: Cell culture Human dermal lymphatic endothelial cells (HLEC) (HMVEC-dLyAd) were purchased from Lonza, and were cultured in EGM-1 MV media on cell culture dishes coated with fibronectin (10 μg/ml) as we described previously 9 .

    Techniques: Western Blot, Control, Fluorescence

    SVEC (A, B) or HLEC (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.

    Journal: Anti-cancer agents in medicinal chemistry

    Article Title: Novel action and mechanism of auranofin in inhibition of vascular endothelial growth factor receptor-3-dependent lymphangiogenesis

    doi:

    Figure Lengend Snippet: SVEC (A, B) or HLEC (C, D) were treated with ARF at 0–4 μM for 4 h. A and C. Cell lysates were subjected to immunoblotting for TrxR2, Trx2, NF-κB, phosphor- and total p38MAPK, and VEGFR3 with respective antibodies. β-actin was used as a loading control. Normalized protein levels are indicated below the blot with untreated as 1.0. Relative ratios of p-p38MAPK and total p38MAPK are also indicated. Similar results were obtained from additional two experiments. B and D. ROS levels from ARF-treated SVEC were measured by Dihydrorhodamine 123 probe in a fluorescence plate reader for 120 min. Data presented are mean±SEM from triplicates of three independent experiments. *, P<0.05; **, P<0.01.

    Article Snippet: Human dermal lymphatic endothelial cells (HLEC) (HMVEC-dLyAd) were purchased from Lonza, and were cultured in EGM-1 MV media on cell culture dishes coated with fibronectin (10 μg/ml) as we described previously 9 .

    Techniques: Western Blot, Control, Fluorescence